Isolation of Vacuoles from Root Storage Tissue of Beta vulgaris L.
نویسندگان
چکیده
منابع مشابه
Isolation of protoplasts and vacuoles from storage tissue of red beet.
A fast and efficient method is described for the isolation of protoplasts and vacuoles from storage tissue of Beta vulgaris L. The viability of the isolated protoplasts is indicated by the development within a few hours of plasma strands with active cyclosis as well as by transport activity.The effect of aging of the tissue on the yield and on the properties of the protoplasts is examined. Addi...
متن کاملEnzymes Involved in the Postharvest Degradation of Sucrose in Beta vulgaris L. Root Tissue.
The reducing sugar content of sugar beet (Beta vulgaris L.) roots increased during 30 days of storage at 21 C and 160 days at 5 C as a result of an increase in acid invertase activity. Sucrose synthetase and neutral invertase activities were high at harvest but declined during storage, thus showing no relationship with postharvest reducing sugar accumulation in sugar beet roots. Acid alpha-gluc...
متن کاملEffect of Trimethyltin Chloride on Slow Vacuolar (SV) Channels in Vacuoles from Red Beet (Beta vulgaris L.) Taproots
In the present study, patch-clamp techniques have been used to investigate the effect of trimethyltin chloride (Met3SnCl) on the slow vacuolar (SV) channels in vacuoles from red beet (Beta vulgaris L.) taproots. Activity of SV channels has been measured in whole-vacuole and cytosolic side-out patch configurations. It was found that addition of trimethyltin chloride to the bath solution suppress...
متن کاملSugar beet (Beta vulgaris L.).
Creating transgenic plants is invaluable for the genetic analysis of sugar beet and will be increasingly important as sugar beet genomic technologies progress. A protocol for Agrobacterium-mediated transformation of sugar beet is described in this chapter. Our protocol is optimized for a sugar beet genotype that performs exceptionally well in tissue culture, including the steps of dedifferentia...
متن کاملThe location of acid invertase activity and sucrose in the vacuoles of storage roots of beetroot (Beta vulgaris).
Vacuoles were isolated from freshly cut slices of the storage roots of beetroot (Beta vulgaris), and from slices that had been washed in aerated water for 1-3 days. The unique vacuolar location of betanin permitted the use of a correlative method to determine whether sucrose and acid invertase were located in the vacuoles. The specific content (the activity of the enzyme or amount of substrate ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1976
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.58.5.656